5MN6

S. aureus FtsZ 12-316 F138A GDP Closed form (3FCm)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.93
Spacegroup name	P 21 21 21
Unit cell lengths	41.121, 68.152, 207.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.200
R-factor	0.22048
Rwork	0.216
R-free	0.29980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mn5
RMSD bond length	0.010
RMSD bond angle	1.573
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.132	1.107
Number of reflections	10264
<I/σ(I)>	10.6	1.7
Completeness [%]	99.9	100
Redundancy	9.9	8.8
CC(1/2)	0.998	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	10 mg/ml; 12.5 % w/v PEG 3,350, 12.5 % w/v PEG 1k, 12.5 % v/v MPD, pH 6.5, 0.1M MES/imidazole, 0.03 M NaF, NaBr and NaI