5MMY
Crystal structure of OXA10 with HEPES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-28 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.772, 102.189, 127.409 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.410 - 1.880 |
Rwork | 0.178 |
R-free | 0.19790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fq9 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.17) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 79.720 | 79.720 | 1.930 |
High resolution limit [Å] | 1.880 | 8.410 | 1.880 |
Rmerge | 0.176 | 0.064 | 2.092 |
Rmeas | 0.183 | ||
Rpim | 0.051 | ||
Total number of observations | 679292 | ||
Number of reflections | 52703 | ||
<I/σ(I)> | 11.8 | 23.7 | 3.2 |
Completeness [%] | 100.0 | 100 | 99.8 |
Redundancy | 12.9 | 11.5 | 12.9 |
CC(1/2) | 0.997 | 0.998 | 0.862 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1M HEPES pH 7.0, 20% PEG 6000 |