5MMX
ABA RECEPTOR FROM CITRUS, CSPYL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 82.613, 82.613, 126.674 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.941 - 2.882 |
| R-factor | 0.2295 |
| Rwork | 0.226 |
| R-free | 0.29330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mmq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.619 |
| Data scaling software | Aimless (v1.7) |
| Phasing software | MOLREP (v1.7) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmeas | 0.424 | |
| Total number of observations | 233833 | |
| Number of reflections | 10428 | |
| <I/σ(I)> | 8.19 | 0.8 |
| Completeness [%] | 96.2 | 99.3 |
| Redundancy | 20 | 21.5 |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290.15 | 0.1M Cacodil. Na pH 6.5, 0.2M Ac.Calc, 40% PEG 300 |
