5MLX
Open loop conformation of PhaZ7 Y105E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8123 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.300, 199.500, 44.400 |
| Unit cell angles | 90.00, 114.20, 90.00 |
Refinement procedure
| Resolution | 23.082 - 1.600 |
| R-factor | 0.1273 |
| Rwork | 0.126 |
| R-free | 0.15200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4brs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.882 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.100 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.130 | |
| Number of reflections | 77568 | |
| <I/σ(I)> | 18.6 | 7.1 |
| Completeness [%] | 89.9 | 91.2 |
| Redundancy | 2.8 | 2.2 |
| CC(1/2) | 0.998 | 0.959 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 0.2 M NH4Cl, O.1 M sodium acetate, 20% PEG6000 |
