5MLW
Crystal structure of human PCNA in complex with ZRANB3 APIM motif peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.2822 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 84.631, 84.631, 201.770 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 73.290 - 2.450 |
R-factor | 0.22308 |
Rwork | 0.221 |
R-free | 0.26420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vym |
RMSD bond length | 0.011 |
RMSD bond angle | 1.420 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.290 | 2.550 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.104 | 1.297 |
Number of reflections | 31686 | |
<I/σ(I)> | 15.1 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.2 | 9.7 |
CC(1/2) | 0.998 | 0.565 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100 mM lithium sulfate + 30% (w/v) polyvinylpyrrolidone + 100 mM HEPES pH 7.0 |