5MKT
Crystal structure of mouse prorenin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-15 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | I 41 |
Unit cell lengths | 141.232, 141.232, 82.961 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.210 - 3.200 |
R-factor | 0.26499 |
Rwork | 0.263 |
R-free | 0.29723 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4amt |
RMSD bond length | 0.006 |
RMSD bond angle | 1.012 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.250 | 3.420 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.088 | 0.739 |
Number of reflections | 71150 | |
<I/σ(I)> | 9.5 | 1.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.2 | 5.6 |
CC(1/2) | 0.998 | 0.827 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 0.75 K/Na H2PO4, 0.1M HEPES PH 7.9 |