5MKM

Crystal Structure of Two-Domain Laccase mutant H165F from Streptomyces griseoflavus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.980583
Spacegroup name	P 1 21 1
Unit cell lengths	75.884, 95.075, 116.821
Unit cell angles	90.00, 90.58, 90.00

Refinement procedure

Resolution	49.767 - 1.996
R-factor	0.2004
Rwork	0.200
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lhl
RMSD bond length	0.008
RMSD bond angle	0.928
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	1.996	1.996
Number of reflections	206927
<I/σ(I)>	5.41	1.49
Completeness [%]	80.2	78.9
Redundancy	2.29	2.06
CC(1/2)	0.990	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	291	20% PEG 6000, 0.1 M Bicine