5MKL
Crystal structure of SmAP (LSm) protein from Sulfolobus acidocaldarius
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.953720 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.622, 133.516, 135.774 |
| Unit cell angles | 86.18, 85.78, 89.94 |
Refinement procedure
| Resolution | 45.034 - 2.086 |
| R-factor | 0.1841 |
| Rwork | 0.182 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xq3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.875 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.085 | 2.085 |
| Rmerge | 0.069 | 0.941 |
| Number of reflections | 148188 | |
| <I/σ(I)> | 9.9 | 1.3 |
| Completeness [%] | 94.7 | 70.3 |
| Redundancy | 4 | 3.7 |
| CC(1/2) | 0.997 | 0.444 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 8% Tacsimate pH 4.0, 20% PEG 3350 |
