5MKA

Maltodextrin binding protein MalE1 from L. casei BL23 bound to gamma-cyclodextrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	54.620, 82.602, 84.847
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.423 - 1.149
R-factor	0.1254
Rwork	0.125
R-free	0.14550
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.159
Data reduction software	XDS (VERSION November 3, 2014)
Data scaling software	XDS (VERSION November 3, 2014)
Phasing software	SHELXDE (2013/2)
Refinement software	PHENIX ((1.10.1-2155_1168: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.930	1.190
High resolution limit [Å]	1.149	1.149
Rmerge	0.087	1.261
Number of reflections	131510
<I/σ(I)>	21.9	2
Completeness [%]	96.1	92.74
Redundancy	13.46	13.31
CC(1/2)	1.000	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 mM Na-Hepes, pH 7.0, 20-25% Jeffamine ED2003, pH 7.0, 5 mM gamma-cyclodextrin