5MK3
Crystal structure of the His Domain Protein Tyrosine Phosphatase (HD-PTP/PTPN23) Bro 1 domain (CHMP4C peptide complex structure)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-01-31 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 |
Unit cell lengths | 66.436, 70.835, 79.291 |
Unit cell angles | 90.54, 98.22, 106.82 |
Refinement procedure
Resolution | 49.920 - 2.000 |
R-factor | 0.1982 |
Rwork | 0.198 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rau |
RMSD bond length | 0.002 |
RMSD bond angle | 0.535 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11_2563: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.920 | 2.071 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.057 | 0.228 |
Number of reflections | 88613 | |
<I/σ(I)> | 11.96 | 4.22 |
Completeness [%] | 95.9 | 94.24 |
Redundancy | 2.2 | 2.1 |
CC(1/2) | 0.996 | 0.949 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M Potassium thiocyanate, 0.1 M Bis-Tris propane pH 6.5, 20% PEG 3350 |