5MJY

Crystal structure of the His Domain Protein Tyrosine Phosphatase (HD-PTP/PTPN23) Bro1 domain (SARA complex structure)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 1
Unit cell lengths	66.330, 70.360, 83.480
Unit cell angles	111.48, 90.06, 93.00

Refinement procedure

Resolution	65.368 - 2.250
R-factor	0.1892
Rwork	0.187
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rau
RMSD bond length	0.002
RMSD bond angle	0.487
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2563: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.368	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.098	0.328
Number of reflections	63820
<I/σ(I)>	6.79	2.83
Completeness [%]	95.8	93.75
Redundancy	2.1	2.2
CC(1/2)	0.981	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	0.1 M MMT buffer pH 9.0, 25% PEG1500