5MJW
Structure of Psb29 at 1.55A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97630 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.850, 86.610, 116.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.260 - 2.470 |
| R-factor | 0.21913 |
| Rwork | 0.218 |
| R-free | 0.24978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mjo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.191 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.260 | 2.530 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.062 | 0.830 |
| Number of reflections | 11662 | |
| <I/σ(I)> | 6.2893 | 0.67 |
| Completeness [%] | 99.6 | 98.77 |
| Redundancy | 6.34 | 6.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.2M sodium malonate, pH 7, 20% w/v/ PEG 3350 |
