5MJP
Multi-bunch pink beam serial crystallography: Phycocyanin (One chip)
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2016-07-10 |
Detector | RAYONIX MX340-HS |
Wavelength(s) | 1.15-1.25 |
Spacegroup name | H 3 2 |
Unit cell lengths | 187.800, 187.800, 60.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.900 - 2.110 |
R-factor | 0.172 |
Rwork | 0.169 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jbo |
RMSD bond length | 0.003 |
RMSD bond angle | 0.828 |
Data reduction software | Precognition |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.920 | 2.220 |
High resolution limit [Å] | 2.106 | 2.120 |
Rmerge | 0.061 | |
Number of reflections | 15458 | 9048 |
<I/σ(I)> | 31.68 | |
Completeness [%] | 64.7 | 26.6 |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | Ammonium sulphate |