5MJO
Structure of Psb29 at 1.55A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91730 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.240, 56.730, 47.730 |
| Unit cell angles | 90.00, 104.72, 90.00 |
Refinement procedure
| Resolution | 46.160 - 1.550 |
| R-factor | 0.13631 |
| Rwork | 0.133 |
| R-free | 0.19243 |
| Structure solution method | SAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.070 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.160 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.114 | 1.098 |
| Number of reflections | 23219 | |
| <I/σ(I)> | 5.2976 | 0.65 |
| Completeness [%] | 99.0 | 99.17 |
| Redundancy | 16.9 | 16.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 290 | 0.1 M Bicine, pH 9.0, 20% w/v PEG 6000 |
