5MJM
Single-shot pink beam serial crystallography: Phycocyanin (Five chips merged)
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2016-07-09 |
| Detector | RAYONIX MX340-HS |
| Wavelength(s) | 1.15-1.25 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 187.800, 187.800, 60.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.917 - 2.302 |
| R-factor | 0.1428 |
| Rwork | 0.139 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jbo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.903 |
| Data reduction software | Epinorm |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.920 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.057 | |
| Number of reflections | 12168 | |
| <I/σ(I)> | 47.2 | |
| Completeness [%] | 68.5 | 0.23 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 293 | Ammonium sulphate |
