5MIX

Extracellular domain of human CD83 - trigonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918409
Spacegroup name	P 3 2 1
Unit cell lengths	62.942, 62.942, 40.064
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.060 - 1.701
R-factor	0.1912
Rwork	0.188
R-free	0.21640
RMSD bond length	0.006
RMSD bond angle	1.088
Data reduction software	XDS
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.060	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.046	0.851
Number of reflections	10348
<I/σ(I)>	23.8	2.6
Completeness [%]	99.9	99.9
Redundancy	9.9	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.4 microliter protein (39 mg/ml in 50 mM Tris-HCl (pH 8.0) buffer) were mixed with 0.2 microliter reservoir solution (1 M ammonium sulfate, 0.1 M HEPES (pH 7.0), 0.5% w/v polyethylene glycol (PEG) 8000) and equilibrated against 70 microliter of reservoir solution