5MIN
Apo form of the soluble PQQ-dependent Glucose Dehydrogenase from Acinetobacter calcoaceticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-03 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.407, 54.898, 85.474 |
| Unit cell angles | 88.04, 81.58, 69.62 |
Refinement procedure
| Resolution | 25.730 - 1.760 |
| R-factor | 0.1795 |
| Rwork | 0.178 |
| R-free | 0.21440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c9u |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XSCALE |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.730 |
| High resolution limit [Å] | 1.760 |
| Number of reflections | 83379 |
| <I/σ(I)> | 9.42 |
| Completeness [%] | 91.6 |
| Redundancy | 3.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 4000 (12%) 100 mM TRIS pH 8.5 |
