5MHG
Crystal structure of anti-T4 Fab fragment with tetraiodoBPA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 131.767, 56.992, 61.993 |
Unit cell angles | 90.00, 98.39, 90.00 |
Refinement procedure
Resolution | 20.090 - 2.801 |
R-factor | 0.2245 |
Rwork | 0.221 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mhe |
RMSD bond length | 0.001 |
RMSD bond angle | 0.353 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.090 | 2.970 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 11247 | |
<I/σ(I)> | 6.7 | 1.21 |
Completeness [%] | 98.8 | 95.5 |
Redundancy | 5 | 4.9 |
CC(1/2) | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M Ammonium sulfate, 25 % PEG 3350, 0.1 M 3350 |