5MH2
Crystal structure of a DM9 domain containing protein from Crassostrea gigas with D22A mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.99999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.160, 56.530, 105.530 |
Unit cell angles | 90.00, 93.41, 90.00 |
Refinement procedure
Resolution | 19.600 - 1.300 |
R-factor | 0.147 |
Rwork | 0.145 |
R-free | 0.18200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mh0 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.577 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 106083 | |
<I/σ(I)> | 17.4 | 2.5 |
Completeness [%] | 98.9 | 96.1 |
Redundancy | 6.3 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M HEPES, 30% PEG 3350, 0.12M Magnisium Chloride hexahydrate |