5MG8

Crystal structure of the S.pombe Smc5/6 hinge domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	172.564, 196.455, 122.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.583 - 2.750
R-factor	0.2108
Rwork	0.209
R-free	0.24940
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.562
Data reduction software	XDS (0.48 (24-Nov-2014))
Data scaling software	Aimless (0.5.14)
Phasing software	SHELXCD
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.583	2.860
High resolution limit [Å]	2.750	2.750
Rmerge	0.123	1.540
Rpim	0.056	0.979
Number of reflections	104437
<I/σ(I)>	11.9	1.4
Completeness [%]	100.0	100
Redundancy	6.6	6.7
CC(1/2)	0.998	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100mM Bis-Tris pH7.5, 2.1M ammonium sulphate