5MG7
New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-p53R2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97630 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 137.621, 49.804, 68.089 |
Unit cell angles | 90.00, 93.34, 90.00 |
Refinement procedure
Resolution | 27.768 - 1.450 |
R-factor | 0.1724 |
Rwork | 0.171 |
R-free | 0.20210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDBID 2AC0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.170 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.6.1) |
Refinement software | PHENIX (dev_2264) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.480 |
High resolution limit [Å] | 1.450 | 3.940 | 1.450 |
Rmerge | 0.091 | 0.048 | 1.011 |
Number of reflections | 82061 | ||
<I/σ(I)> | 6.6 | ||
Completeness [%] | 99.9 | 99.2 | 98.5 |
Redundancy | 6 | 6.2 | 4.4 |
CC(1/2) | 0.998 | 0.804 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 292 | 0.07 M ammonium tartrate dibasic, 8.4% Polyethylene glycol 3,350 |