5MG0
Structure of PAS-GAF fragment of Deinococcus phytochrome by serial femtosecond crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE MFX |
| Synchrotron site | SLAC LCLS |
| Beamline | MFX |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2016-09-30 |
| Detector | RAYONIX MX170-HS |
| Wavelength(s) | 1.265 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.535, 53.385, 80.912 |
| Unit cell angles | 90.00, 116.32, 90.00 |
Refinement procedure
| Resolution | 32.243 - 1.650 |
| R-factor | 0.1737 |
| Rwork | 0.172 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 2O9C lacking the bilin and all neighboring side chains |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.954 |
| Data reduction software | cctbx.prime |
| Data scaling software | cctbx.prime |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 72.500 | 72.524 | 1.709 |
| High resolution limit [Å] | 1.650 | 3.555 | 1.650 |
| Number of reflections | 41877 | ||
| <I/σ(I)> | 62.83 | 371.677 | 2.414 |
| Completeness [%] | 100.0 | 99.81 | 99.93 |
| Redundancy | 72.62 | 195.78 | 17.05 |
| CC(1/2) | 0.968 | 0.950 | 0.087 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5.6 | 298 | PEG 3350, isopropanol, glycerol, sodium citrate |
