5MFX
Zika NS3 helicase:RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.355, 73.037, 58.696 |
| Unit cell angles | 90.00, 93.97, 90.00 |
Refinement procedure
| Resolution | 45.690 - 1.598 |
| R-factor | 0.1634 |
| Rwork | 0.162 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jlq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.545 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.690 | 1.640 |
| High resolution limit [Å] | 1.598 | 1.598 |
| Rmerge | 0.040 | 0.756 |
| Number of reflections | 57739 | |
| <I/σ(I)> | 14.95 | 1.46 |
| Completeness [%] | 98.8 | 96.2 |
| Redundancy | 3 | 2.86 |
| CC(1/2) | 0.999 | 0.484 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% (w/v) ethylene glycol, 10% (w/v) polyethylene glycol 8000, 0.1 M MES/Imidazole pH 6.5, 20mM sodium formate, 20mM ammonium acetate, 20mM tri-sodium citrate, 20mM sodium potassium L-tartrate. |
