5MFM
Designed armadillo repeat protein peptide fusion YIIIM6AII_GS11_(KR)5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.760, 89.390, 123.850 |
Unit cell angles | 90.00, 97.07, 90.00 |
Refinement procedure
Resolution | 44.690 - 2.300 |
R-factor | 0.219 |
Rwork | 0.217 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.690 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.114 | 1.630 |
Number of reflections | 77719 | |
<I/σ(I)> | 8.67 | 1.07 |
Completeness [%] | 98.6 | 99.1 |
Redundancy | 4.2 | 4.3 |
CC(1/2) | 0.998 | 0.156 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 37.5% (MPD/PEG1000/PEG3350), 0.03 M MgCl2, 0.03 M CaCl2, Bicine/TRIS pH 8.5 |