5MFH
Designed armadillo repeat protein YIIIM5AII in complex with peptide (RR)5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-06 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.480, 103.790, 134.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.390 - 2.000 |
R-factor | 0.2 |
Rwork | 0.199 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mfe |
RMSD bond length | 0.008 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.390 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.543 | 9.230 |
Number of reflections | 77426 | |
<I/σ(I)> | 5.52 | 0.29 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 13.1 | 11 |
CC(1/2) | 0.990 | 0.108 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 277 | 5% PEG8000, 27.27% MPD, 0.1 M Na Cacodylate pH6.7 |