5MFG
Designed armadillo repeat protein YIIIM5AII in complex with peptide (RR)4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-07 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 62 |
| Unit cell lengths | 168.540, 168.540, 80.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.653 - 1.901 |
| R-factor | 0.2075 |
| Rwork | 0.182 |
| R-free | 0.21330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pls |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.496 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.653 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.138 | 6.140 |
| Number of reflections | 102534 | |
| <I/σ(I)> | 19.47 | |
| Completeness [%] | 99.9 | 98.3 |
| Redundancy | 20.6 | 20.5 |
| CC(1/2) | 1.000 | 0.190 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 18.0% PEG 3350, 0.15 M KSCN, 0.1 M Na-Acetate pH5.5 |
