5MFC

Designed armadillo repeat protein YIIIM5AII in complex with (KR)4-GFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	81.110, 124.240, 245.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.560 - 2.400
R-factor	0.219
Rwork	0.218
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5aei 1gfl
RMSD bond length	0.010
RMSD bond angle	1.250
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.560	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.128	4.590
Number of reflections	47804
<I/σ(I)>	15.17	0.58
Completeness [%]	97.9	98.2
Redundancy	12.4	12.8
CC(1/2)		0.174

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	8% w/v PEG 4000, 0.1M Na acetate trihydrate pH 4.6,