5MFB
Designed armadillo repeat protein YIII(Dq)4CqI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-28 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.230, 46.560, 90.810 |
| Unit cell angles | 90.00, 128.55, 90.00 |
Refinement procedure
| Resolution | 42.810 - 2.300 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.810 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.081 | 1.850 |
| Number of reflections | 20111 | |
| <I/σ(I)> | 10.31 | 0.76 |
| Completeness [%] | 97.3 | 97.1 |
| Redundancy | 3.8 | 4 |
| CC(1/2) | 0.999 | 0.298 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.3 M Na-Acetate, 0.1 M Na-Cacodylate pH 6.5, 25 % PEG 2K MME |
