5MF6
Human Sirt6 in complex with activator UBCS039
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 63 |
Unit cell lengths | 91.300, 91.300, 144.317 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.650 - 1.870 |
R-factor | 0.19141 |
Rwork | 0.190 |
R-free | 0.21957 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k35 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.324 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.650 | 1.980 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmeas | 0.140 | 1.320 |
Number of reflections | 56118 | |
<I/σ(I)> | 12.14 | |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 10.3 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 1.6 M (NH4)2SO4, 10% PEG 400, Bis-Tris buffer pH 5.7 |