5MF2
Bacteriophage T5 distal tail protein pb9 co-crystallized with Tb-Xo4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.64 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.464, 95.502, 71.492 |
| Unit cell angles | 90.00, 103.46, 90.00 |
Refinement procedure
| Resolution | 48.380 - 2.000 |
| R-factor | 0.211 |
| Rwork | 0.210 |
| R-free | 0.23600 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.596 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.384 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 122847 |
| <I/σ(I)> | 9.5 |
| Completeness [%] | 99.4 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 6 to 11 % PEG 6000 and MPD 5% in 100 mM HEPES buffer pH 7.5 |
