5MEI
Crystal structure of Agelastatin A bound to the 80S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.148 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 304.080, 286.550, 436.550 |
| Unit cell angles | 90.00, 99.05, 90.00 |
Refinement procedure
| Resolution | 98.383 - 3.500 |
| R-factor | 0.1962 |
| Rwork | 0.195 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v88 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 98.383 | 100.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 98.383 | 3.500 |
| Rmerge | 0.567 | 2.259 | |
| Rmeas | 0.597 | 2.425 | |
| Total number of observations | 9768849 | ||
| Number of reflections | 925728 | 74772 | |
| <I/σ(I)> | 5.56 | 1 | |
| Completeness [%] | 100.0 | 99.9 | |
| Redundancy | 10.553 | 7.424 | |
| CC(1/2) | 0.985 | 0.322 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine |
