5MEE
Cyanothece lipoxygenase 2 (CspLOX2) variant - L304V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.710, 167.100, 167.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.350 - 2.500 |
R-factor | 0.194 |
Rwork | 0.193 |
R-free | 0.22000 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5med |
RMSD bond length | 0.007 |
RMSD bond angle | 0.761 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.350 | 2.600 | |
High resolution limit [Å] | 2.500 | 50.000 | 2.500 |
Rmerge | 0.048 | 0.600 | |
Number of reflections | 53895 | ||
<I/σ(I)> | 22.68 | 3.02 | |
Completeness [%] | 99.7 | 99.9 | |
Redundancy | 5.7 | ||
CC(1/2) | 0.999 | 0.859 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 15% PEG 4000, 10% Glycerol, 0.1 M MES/immidazol pH 6.1 |