5MEA
Crystal structure of yeast Cdt1 (N terminal and middle domain), form 2.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.510, 89.350, 121.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.948 - 2.152 |
R-factor | 0.2056 |
Rwork | 0.203 |
R-free | 0.24910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | the structure i'm depositing with this one - that was solved by SAD (SeMet) - sorry i don't have a pdb code yet... |
RMSD bond length | 0.002 |
RMSD bond angle | 0.606 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 2.260 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.098 | 1.060 |
Number of reflections | 28350 | |
<I/σ(I)> | 13.1 | 2 |
Completeness [%] | 98.7 | 95.4 |
Redundancy | 5.8 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 10.5% PEG 8K, 18% glycerol, 0.5M lithium sulphate |