5MDT

Structure of the CTD-interacting domain (CID) of Seb1 from S. pombe.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 31 2 1
Unit cell lengths	55.560, 55.560, 131.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.120 - 1.620
Rwork	0.195
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3clj
RMSD bond length	0.010
RMSD bond angle	0.980
Data reduction software	xia2
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.120	1.660
High resolution limit [Å]	1.620	1.620
Rmerge	0.098	2.151
Number of reflections	30490
<I/σ(I)>	14.2	1.1
Completeness [%]	99.3	98.5
Redundancy	8.5	4.8
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.65	100 mM Tris, 4 M NaCl