5MCW
New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-LWC2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.88560 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 111.403, 68.408, 69.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.815 - 1.897 |
R-factor | 0.2024 |
Rwork | 0.200 |
R-free | 0.23480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tsr |
RMSD bond length | 0.006 |
RMSD bond angle | 0.867 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX (dev-2276_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.897 | 5.160 | 1.897 |
Rmerge | 0.143 | 0.098 | 0.305 |
Number of reflections | 40479 | ||
<I/σ(I)> | 3.4 | ||
Completeness [%] | 94.5 | 99.3 | 75.7 |
Redundancy | 6.1 | 6 | 5.2 |
CC(1/2) | 0.990 | 0.954 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.8 | 292 | 0.02 M Citric acid, 0.08 M BIS-TRIS propane, 18% w/v Polyethylene glycol 3,350 |