5MBK
Structure of a bacterial light-regulated adenylyl cylcase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 69.600, 143.360, 57.480 |
Unit cell angles | 90.00, 135.91, 90.00 |
Refinement procedure
Resolution | 45.880 - 2.400 |
R-factor | 0.2117 |
Rwork | 0.209 |
R-free | 0.26380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5m27 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.357 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2196) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.900 | 2.400 | |
High resolution limit [Å] | 2.300 | 10.000 | 2.300 |
Rmerge | 0.103 | 0.047 | 0.983 |
Number of reflections | 17359 | ||
<I/σ(I)> | 10.29 | 31.85 | 1.84 |
Completeness [%] | 98.5 | 94.3 | 98.7 |
Redundancy | 5.2 | ||
CC(1/2) | 0.997 | 0.997 | 0.682 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.1 M Na-HEPES pH 7.0, 20 % w/v PEG 3350; soak in 50mM Tris-HCl pH 7.6, 115 mM MgCl2, 48 mM ATP, 35% (w/v) PEG 3350 before cryo-cooling |