5MBJ
Structure of a bacterial light-regulated adenylyl cyclase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.954 |
Spacegroup name | P 1 |
Unit cell lengths | 47.660, 57.070, 71.310 |
Unit cell angles | 82.42, 75.79, 76.88 |
Refinement procedure
Resolution | 45.244 - 2.300 |
R-factor | 0.2045 |
Rwork | 0.203 |
R-free | 0.25280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5m27 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.539 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.250 | 2.400 | |
High resolution limit [Å] | 2.300 | 10.000 | 2.300 |
Rmerge | 0.059 | 0.021 | 0.698 |
Rmeas | 0.083 | 0.030 | 0.987 |
Total number of observations | 53661 | ||
Number of reflections | 30125 | 361 | 3590 |
<I/σ(I)> | 8.46 | 34.09 | 1.09 |
Completeness [%] | 95.9 | 93.3 | 95.8 |
Redundancy | 1.8 | ||
CC(1/2) | 0.997 | 0.998 | 0.440 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.1 M Tris-HCl pH 8.0, 0.3 M Mg(NO3)2, 23 % w/v PEG 2000; Cryoprotection in 0.1 M Tris HCl pH 8.0, 50 mM MgCl2, 35 % w/v PEG 3350 |