5MBG
Structure of a bacterial light-regulated adenylyl cyclase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-08-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 50.720, 101.790, 80.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.397 - 2.300 |
R-factor | 0.2431 |
Rwork | 0.240 |
R-free | 0.29250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5m27 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.354 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.397 | 2.400 | |
High resolution limit [Å] | 2.300 | 10.000 | 2.300 |
Rmerge | 0.108 | 0.048 | 1.099 |
Rmeas | 0.112 | 0.051 | 1.146 |
Total number of observations | 118071 | ||
Number of reflections | 9537 | 141 | 1114 |
<I/σ(I)> | 17.42 | 36.52 | 3.5 |
Completeness [%] | 99.8 | 99.3 | 99.2 |
Redundancy | 12.4 | ||
CC(1/2) | 0.999 | 0.999 | 0.872 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.1 M Na-HEPES pH 7.0, 0.2 M NaI, 20 % w/v PEG 3350 |