5MBC
Structure of a bacterial light-regulated adenylyl cylcase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-04-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 47.950, 56.670, 78.090 |
Unit cell angles | 104.20, 104.01, 102.54 |
Refinement procedure
Resolution | 44.549 - 1.800 |
R-factor | 0.1909 |
Rwork | 0.190 |
R-free | 0.22530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5m27 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.441 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.550 | 44.550 | 1.900 |
High resolution limit [Å] | 1.800 | 10.000 | 1.800 |
Rmerge | 0.090 | 0.068 | 0.403 |
Rmeas | 0.108 | 0.079 | 0.570 |
Total number of observations | 179197 | ||
Number of reflections | 65175 | 391 | 9367 |
<I/σ(I)> | 10.04 | 24.99 | 1.78 |
Completeness [%] | 94.9 | 97 | 90.9 |
Redundancy | 2.7 | ||
CC(1/2) | 0.992 | 0.987 | 0.716 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1 M Na-MES pH 6.5, 0.6 M NaCl, 20 % w/v PEG 3350; 0.3 mM alpha,beta-methylene-ATP in drop |