5MB9

Crystal structure of the eukaryotic ribosome associated complex (RAC), a unique Hsp70/Hsp40 pair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9733
Spacegroup name	C 2 2 21
Unit cell lengths	93.918, 179.359, 155.455
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.973 - 3.200
R-factor	0.2301
Rwork	0.227
R-free	0.28490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4GNI AND 1DKZ
RMSD bond length	0.003
RMSD bond angle	0.703
Data reduction software	xia2
Data scaling software	Aimless (0.2.17)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.973	47.970	3.420
High resolution limit [Å]	3.200	9.050	3.200
Rmerge	0.150	0.042	1.269
Number of reflections	21953
<I/σ(I)>	9
Completeness [%]	99.6	99.3	100
Redundancy	5.2	4.5	5.6
CC(1/2)	0.997	0.998	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1 M HEPES pH 7.5, 26% PEG 3350 and 0.2 M Ammonium acetate