5MB7
Cocktail experiment B: fragments 224 and 236 at 50mM concentration
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-24 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.398, 72.858, 52.710 |
| Unit cell angles | 90.00, 109.46, 90.00 |
Refinement procedure
| Resolution | 18.926 - 1.299 |
| R-factor | 0.1304 |
| Rwork | 0.129 |
| R-free | 0.14970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y5l |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.908 |
| Data reduction software | HKL-2000 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.052 | 0.520 |
| Number of reflections | 78870 | |
| <I/σ(I)> | 22.3 | 2 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 290 | 0.1 M ammonium acetate, 0.1 M sodium acetate, PEG 4000; crystals obtained by streak-seeding |
