5MB2

Structure of sucrose phosphorylase from Bifidobacterium adolescentis bound to nigerose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97625
Spacegroup name	P 43 21 2
Unit cell lengths	82.915, 82.915, 153.644
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.607 - 1.752
R-factor	0.1991
Rwork	0.197
R-free	0.23220
RMSD bond length	0.004
RMSD bond angle	0.623
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_2443)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.607
High resolution limit [Å]	1.752
Number of reflections	54566
<I/σ(I)>	24.36
Completeness [%]	99.9
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	PEG 1000, 150mM NaCl, MES, 2% Sacharose