5MAU
Crystal structure of dimeric chlorite dismutase from Cyanothece sp. PCC7425 (pH 6.5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 51.120, 52.650, 54.730 |
Unit cell angles | 107.22, 99.22, 108.90 |
Refinement procedure
Resolution | 46.380 - 1.300 |
R-factor | 0.148 |
Rwork | 0.147 |
R-free | 0.17600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qpi |
RMSD bond length | 0.017 |
RMSD bond angle | 1.481 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (DEV_2712) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.381 | 1.346 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.080 | 2.070 |
Number of reflections | 115659 | |
<I/σ(I)> | 10.55 | 0.99 |
Completeness [%] | 95.0 | 91.81 |
Redundancy | 6.8 | 7 |
CC(1/2) | 0.998 | 0.399 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.15 M MgSO4, 0.1 M MES pH 6.5, 25 % (w/v) PEG 3350 |