5MA8

GFP-binding DARPin 3G124nc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00002
Spacegroup name	P 41
Unit cell lengths	62.140, 62.140, 213.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.940 - 2.350
R-factor	0.1916
Rwork	0.189
R-free	0.23580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.940
High resolution limit [Å]	2.350
Number of reflections	33548
<I/σ(I)>	22.33
Completeness [%]	99.9
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.3 M Sodium Acetate. 0.1M TRIS (HOAc) pH 7.5, 25% PEG 2K MME