5M9X
Structure of sucrose phosphorylase from Bifidobacterium adolescentis bound to glycosylated resveratrol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 83.103, 83.103, 157.209 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.065 - 2.349 |
R-factor | 0.2598 |
Rwork | 0.258 |
R-free | 0.28480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.623 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2443: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.000 |
High resolution limit [Å] | 2.349 |
Number of reflections | 23334 |
<I/σ(I)> | 20.88 |
Completeness [%] | 98.0 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 289 | PEG 1000, 150MM NACL, MES, 2% |