5M9O
Crystal structure of human SND1 extended Tudor domain in complex with a symmetrically dimethylated E2F peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.243, 80.626, 39.289 |
Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
Resolution | 28.140 - 1.450 |
R-factor | 0.23039 |
Rwork | 0.229 |
R-free | 0.26120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3omc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.539 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.140 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.037 | 0.292 |
Number of reflections | 38367 | |
<I/σ(I)> | 16.2 | 2.8 |
Completeness [%] | 96.0 | 69.9 |
Redundancy | 3.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 10 % PEG 1000, 10% PEG 8000 |