5M9A
Human angiogenin PD variant H13R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 37.379, 85.606, 32.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.800 - 1.950 |
| R-factor | 0.23897 |
| Rwork | 0.232 |
| R-free | 0.29676 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ang |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.407 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 8160 | |
| <I/σ(I)> | 4.9 | 1.2 |
| Completeness [%] | 99.2 | 91.2 |
| Redundancy | 4.3 | 4 |
| CC(1/2) | 0.986 | 0.466 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M Na/K tartrate 20% PEG 3350 |
