5M94
Crystal structure of Staphylococcus capitis divalent metal ion transporter (DMT) in complex with nanobody
Replaces: 4WGVExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 114.350, 114.350, 257.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.931 - 3.101 |
| R-factor | 0.2391 |
| Rwork | 0.237 |
| R-free | 0.27340 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.505 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.060 | 1.200 |
| Number of reflections | 35853 | |
| <I/σ(I)> | 12 | 2.1 |
| Completeness [%] | 99.6 | 98.2 |
| Redundancy | 9.5 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 50 mM Hepes pH 7.4, 200 mM CaCl2 22-26 % PEG400 |
