5M8L

Crystal structure of human tyrosinase related protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	90.424, 141.758, 191.741
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.466 - 2.350
R-factor	0.2161
Rwork	0.214
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nm8
RMSD bond length	0.011
RMSD bond angle	1.107
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.980
High resolution limit [Å]	2.350
Number of reflections	102816
<I/σ(I)>	6.6
Completeness [%]	99.7
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	0.1 M Tris (pH 7.0), 0.2 M NaCl and 30% (w/v) PEG 3000