5M8J
Crystal structure of Eremococcus coleocola manganese transporter mutant H236A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-04-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.8927 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 150.400, 81.790, 96.440 |
Unit cell angles | 90.00, 107.47, 90.00 |
Refinement procedure
Resolution | 11.996 - 3.700 |
R-factor | 0.2127 |
Rwork | 0.210 |
R-free | 0.25690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5m87 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.496 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.800 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmeas | 0.059 | 1.477 |
Number of reflections | 11966 | |
<I/σ(I)> | 17.2 | 1.96 |
Completeness [%] | 98.9 | 99.9 |
Redundancy | 11.2 | |
CC(1/2) | 0.999 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 50 mM Tris-HCl pH 8.2 22.6% PEG 400 (v/v) |